Exposure assessment of salmonella related to pork handling and consumption at households in Hung Yen and Nghe An provinces

Ngan Thi Tran, Sinh Xuan Dang, Fred Unger


Introduction: Along with poultry and eggs, pork meat represents one of the most signifi cant risks of Salmonellosis. Pork accounts for 56% of the meat consumed daily in Vietnamese households and pork preparation and handling poses a major risk due to possible cross-contamination. The objective of this study is to conduct the exposure assessment by determination of Salmonella prevalence, concentration in market purchased pork and pork handling, and consumption at households in term of food safety in Hung Yen and Nghe An provinces.
Methods: A total of 217 pork samples were collected from pork shops in wet markets for Salmonella analysis between April 2014 and February 2015. A cross-sectional survey on pork
handling and consumption at households was also conducted using a structured questionnaire in 416 households in these two provinces.
Results: Overall Salmonella prevalence in market pork was 44.7% with an average concentration of 9 (<0.3 to >110) MPN/g. Most of consumers purchased pork at the market in the morning around 7:30 am with an average purchase amount of 0.45 (0.1-9.5) kg pork/ time. The methods used for storing raw pork before cooking were at room temperature (54.6%, 228/416) or in a refrigerator (41.8%, 174/416)) with an average duration of 0.6 and 2.4 hours, respectively. The percentages of household using the same knife or cutting board to prepare both raw and cooked pork were 80.5% and 74.5%, respectively.

Conclusion: These fi ndings highlight the high level of Salmonella in pork sold at informal retail markets. This study also shows that the behavior and practices of Vietnamese consumers can present a risk of Salmonella cross-contamination in the household that required the training and education programs on food safety practice of consumer. 


Pork; Salmonella; consumer; household; retail market

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Cox LA, Jr., Ricci PF. Causal regula ons vs. poli cal will: why human zoono c infec ons increase despite precau onary bans on animal an bio cs. Environ Int. May 2008;34(4):459-475.

2008b E. Opinion of the Panel on Biological Hazards on a request from the European Commission on a quan ta ve microbiological risk assessment on Salmonella in meat: Source a ribu on for human salmonellosis from meat. The EFSA Journal. 2008:1-32.

Majowicz SE, Musto J, Scallan E, et al. The global burden of nontyphoidal Salmonella gastroenteri s. Clin Infect Dis. Mar 15 2010;50(6):882-889.

VFA. 2010 -2015: food poisoning record 2016.

WHO. The world health report. 2007; h p://www. who.int/whr/2007/en/index.html. Accessed 15 July, 2016.

2008a E. The community Summary Report: Trends and Sources of Zonoses, Zono c Agents, An microbial resistance and Foodborne outbreaks in European Union in 2008. The EFSA Journal. 2008.

OECD. Meat consump on (indicator). 2016; h ps://data.oecd.org/agroutput/meat-consump on. htm. Accessed March 1, 2016.

4833-1:2002 T. Meat and meat produc on – Sampling and sample test standards. 2002.

2002 I. Interna onal Standard Organiza on: Microbiology of food and animal feed stuff s – Hoizantal method for the detec on of Salmonella spp. 2002.

. JC dM. MPN tables. Eur J Appl Microbiol Biotechnol. 1983;17:301-305.

. Xuan SD, Nguyen-Viet H, Meeyam T, Fries R. Quan fying Salmonella contamina on in pig slaughterhouses in Hung Yen, Vietnam. Paper presented at: the 10th Year Anni- versary of Veterinary Public Health Centre for Asia Pacific 2013-07-02, 2013; Chiang Mai, Thailand.

Phan TT, Khai LT, Ogasawara N, et al. Contamina on of Salmonella in retail meats and shrimps

in the Mekong Delta, Vietnam. J Food Prot. May 2005;68(5):1077-1080.

. Takeshi K, Itoh S, Hosono H, et al. Detec on of Salmonella spp. Isolates from specimens due to pork produc on Chains in Hue City, Vietnam. J Vet Med Sci. Apr 2009;71(4):485-487.

. Thai TH, Yamaguchi R. Molecular characteriza on of an bio c-resistant Salmonella isolates from retail meat from markets in Northern Vietnam. Journal of food protec on. Sep 2012;75(9):17091714.

. Patchanee P, Tansiricharoenkul K, Buawiratlert T, et al. Salmonella in pork retail outlets and dissemina on of its pulsotypes through pig produc on chain in Chiang Mai and surrounding areas, Thailand. Preven ve veterinary medicine. Aug 1 2016;130:99-105.

. Hansen TB, Christensen BB, Aabo S. Salmonella in pork cu ngs in supermarkets and butchers’ shops in Denmark in 2002 and 2006. Zoonoses and public health. Nov 2010;57 Suppl 1:23-29.

. Prendergast DM, Duggan SJ, Gonzales-Barron U, et al. Prevalence, numbers and characteris cs of Salmonella spp. on Irish retail pork. Interna onal journal of food microbiology. May 31 2009;131(23):233-239.

. Delhalle L, Saegerman C, Farnir F, et al. Salmonella surveillance and control at post-harvest in the Belgian pork meat chain. Food microbiology. May 2009;26(3):265-271.

9. Velugo PR, Bohra LK, Juneja VK, et al. Dynamic model for predic ng growth of Salmonella spp. in ground sterile pork. Food microbiology. Jun 2011;28(4):796-803.

0. Lapar M, Tiongco, M. Private standards in pork value chains: Role, impact and poten al for local innova on to improve food safety and enhance smallholder compe veness. Farm Policy Journal. 2011;8(3):39-53.

weather W. World weather online: Vietnam. 2006; h p://www.worldweatheronline.com/vietnamweather.aspx. Accessed 18 June, 2016.

Pin C, Avendano-Perez G, Cosciani-Cunico E, et al. Modelling Salmonella concentra on throughout the pork supply chain by considering growth and survival in fl uctua ng condi ons of temperature, pH and a(w). Interna onal journal of food microbiology. Mar 1 2011;145 Suppl 1:S96-102.

Kusumaningrum HD, van Asselt ED, Beumer RR, Zwietering MH. A quan ta ve analysis of crosscontamina on of Salmonella and Campylobacter spp. via domes c kitchen surfaces. Journal of food protec on. Sep 2004;67(9):1892-1903.

4. van Asselt ED, de Jong AE, de Jonge R, Nauta MJ. Cross-contamina on in the kitchen: es ma on of transfer rates for cu ng boards, hands and knives. Journal of applied microbiology. Nov 2008;105(5):1392-1401.

Sinh DX, Phuc, P. D., Nam, N. H., Thanh, N. T., Hue, V. T. K., Long, N. H., Hung, N. V. Salmonella crosscontamina on of pork for health risk assessment: Simula on of cooking prepara on at households. Vietnamese Journal of Prac cal Medicine. 2014;5(933-934):233-237.

Zhao P, Zhao T, Doyle MP, Rubino JR, Meng J. Development of a model for evalua on of microbial cross-contamina on in the kitchen. Journal of food protec on. Aug 1998;61(8):960-963.

Havelaar AH, Kirk MD, Torgerson PR, et al. World Health Organiza on Global Es mates and Regional Comparisons of the Burden of Foodborne Disease in 2010. PLoS medicine. Dec 2015;12(12):e1001923.